Journal: Pharmaceutical Research

Article Title: Effect of Apolipoprotein E isoforms on the Abundance and Function of P-glycoprotein in Human Brain Microvascular Endothelial Cells

doi: 10.1007/s11095-024-03731-0

Figure Lengend Snippet: Recombinant apoE3 (rE3) and recombinant apoE4 (rE4) do not affect P-gp function as assessed via rhodamine 123 (R123) accumulation after 60 minutes. Treatment with ( a ) rE3 or rE4 (2 µg/mL) for 48 hours prior to functional assay and ( b ) PSC 833 (5 µM) which was used as a positive control for inhibition of P-gp. Data presented as mean ± SEM (n=4) expressed as % R123 accumulation of vehicle control when assessed by ( a ) one-way ANOVA and ( b ) unpaired t-test, ** p< 0.01.

Article Snippet: PSC 833 (Cat.#20391) was purchased from Cayman Chemical (Ann Arbor, MI).

Techniques: Recombinant, Functional Assay, Positive Control, Inhibition, Control

Journal: Cancers

Article Title: A Role for iNOS in Erastin Mediated Reduction of P-Glycoprotein Transport Activity

doi: 10.3390/cancers16091733

Figure Lengend Snippet: Changes in P-glycoprotein (P-gp) transport activity in brain capillaries ( N = 15–20 per data point) from six rats treated with erastin. ( A ) Representative confocal fluorescent and DIC images of brain capillaries after a 60-min incubation with 2.0 μM NBD-CSA; note the high luminal fluorescence in the control capillary and ( B ) decreased luminal fluorescence in capillaries exposed to 10.0 μM PSC833 (Scale bars, 10 μm). Representative confocal images of the luminal fluorescence (LF) from ( C ) male and female brain capillaries associated with P-glycoprotein (P-gp) transport activity at increasing doses of erastin in brain capillaries of SD rats. Bar graphs of P-glycoprotein transport activity determined by LF in ( D ) male and ( E ) female capillaries. Graphs of LF measuring P-glycoprotein (P-gp) transport activity in male ( F ) and female ( G ) capillaries (capillary N = 15–20 per experimental data point) exposed to 0.001 μM erastin over time. Dotted red line denotes multiple comparison. Significance is as compared to control unless otherwise specified: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: PSC833 (valspodar), the P-glycoprotein-specific inhibitor, was purchased from Tocris Bioscience.

Techniques: Activity Assay, Incubation, Fluorescence, Control, Comparison